2OEJ
Crystal structure of a rubisco-like protein from Geobacillus kaustophilus (tetramutant form), liganded with phosphate ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-16 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97811 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 132.673, 132.673, 167.469 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.800 - 2.550 |
| R-factor | 0.233 |
| Rwork | 0.233 |
| R-free | 0.24300 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 2.550 |
| Rmerge | 0.068 |
| Number of reflections | 54519 |
| Completeness [%] | 97.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1.4 M Na/K phosphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
