2OEF
Open and Closed Structures of the UDP-Glucose Pyrophosphorylase from Leishmania major
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.05 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 72.952, 107.661, 150.131 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.950 - 2.400 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.950 | 2.550 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 21635 | |
| Completeness [%] | 92.1 | 83.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 21% PEG 3350, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
