2OEE
yheA from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2006-12-18 |
| Detector | SBC |
| Wavelength(s) | 0.97944 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 53.246, 53.246, 166.348 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.130 - 1.960 |
| R-factor | 0.21829 |
| Rwork | 0.217 |
| R-free | 0.24924 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.345 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.011 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Number of reflections | 19402 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
