2OE9
High-pressure structure of pseudo-WT T4 Lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2004-11-15 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.554, 60.554, 95.567 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 52.410 - 2.010 |
| R-factor | 0.163 |
| Rwork | 0.160 |
| R-free | 0.21000 |
| Structure solution method | ISOMORPHOUS REPLACEMENT |
| Starting model (for MR) | 1l63 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.308 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 60.000 | 60.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.055 | 0.041 | 0.140 |
| Number of reflections | 12985 | ||
| <I/σ(I)> | 23.8 | ||
| Completeness [%] | 90.5 | 89.4 | 38.2 |
| Redundancy | 6.1 | 6 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 293 | ~2.0 M NA/K PHOSPHATES, 50 MM BETA-MERCAPTOETHANOL, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
