2OE7
High-Pressure T4 Lysozyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2004-11-15 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 60.715, 60.715, 96.009 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 52.560 - 2.100 |
R-factor | 0.16 |
Rwork | 0.157 |
R-free | 0.21400 |
Structure solution method | ISOMORPHOUS REPLACEMENT |
Starting model (for MR) | 1l63 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.357 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 60.000 | 60.000 | 2.180 |
High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
Rmerge | 0.041 | 0.029 | 0.115 |
Number of reflections | 11985 | ||
<I/σ(I)> | 22.4 | ||
Completeness [%] | 95.3 | 90.4 | 92.5 |
Redundancy | 7.5 | 7 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 293 | ~2.0 M NA/K PHOSPHATES, 50 MM BETA-MERCAPTOETHANOL, PH 7.1, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |