2OE4

High Pressure Psuedo Wild Type T4 Lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	293
Detector technology	CCD
Collection date	2004-11-15
Detector	ADSC QUANTUM 4
Spacegroup name	P 32 2 1
Unit cell lengths	60.741, 60.741, 96.028
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	52.630 - 2.100
R-factor	0.164
Rwork	0.161
R-free	0.22200
Structure solution method	ISOMORPHOUS REPLACEMENT
Starting model (for MR)	1l63
RMSD bond length	0.016
RMSD bond angle	1.446
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.070	0.202
Number of reflections	12182
<I/σ(I)>	17.9
Completeness [%]	96.8	93.7
Redundancy	6.7	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.1	293	~2.0 M NA/K PHOSPHATES, 50 MM BETA-MERCAPTOETHANOL, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K