2OE2
MSrecA-native-low humidity 95%
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-05-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 |
| Unit cell lengths | 103.013, 103.013, 75.997 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.220 - 3.450 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubc |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.300 | 3.570 |
| High resolution limit [Å] | 3.450 | 3.450 |
| Rmerge | 0.133 | 0.542 |
| Number of reflections | 5573 | |
| <I/σ(I)> | 4.8 | 1.5 |
| Completeness [%] | 90.9 | 94.5 |
| Redundancy | 4.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Citrate phosphote, PEG 4000, Sodium Citrate, NACL, pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
