2ODT
Structure of human Inositol 1,3,4-trisphosphate 5/6-kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-10 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.93 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.330, 39.550, 67.390 |
| Unit cell angles | 90.00, 98.40, 90.00 |
Refinement procedure
| Resolution | 20.220 - 2.010 |
| R-factor | 0.18117 |
| Rwork | 0.177 |
| R-free | 0.25723 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.802 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 20997 | |
| <I/σ(I)> | 11 | 3.4 |
| Completeness [%] | 95.5 | 77.2 |
| Redundancy | 3.4 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | 1.6 M Citrate, Tris buffer, 0.1 M, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
