2ODP
Complement component C2a, the catalytic fragment of C3- and C5-convertase of human complement
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-24 |
| Detector | MAR CCD 225 mm |
| Wavelength(s) | 0.97969 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.398, 83.819, 75.243 |
| Unit cell angles | 90.00, 92.10, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.209 |
| Rwork | 0.207 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2odq |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.637 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.480 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.040 | |
| Number of reflections | 49897 | |
| <I/σ(I)> | 31.7 | 7.1 |
| Completeness [%] | 98.0 | 91.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 20% PEG 10000, 0.1M HEPES, 0.3M glycyl-glycyl-glycine, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
