2ODN
MSRECA-dATP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 2003-01-25 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 61 |
Unit cell lengths | 103.634, 103.634, 77.143 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.920 - 3.100 |
R-factor | 0.231 |
Rwork | 0.231 |
R-free | 0.28500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ubc |
RMSD bond length | 0.009 |
RMSD bond angle | 1.700 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.210 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.084 | 0.510 |
Number of reflections | 8634 | |
<I/σ(I)> | 6.1 | 1.3 |
Completeness [%] | 99.6 | 99.5 |
Redundancy | 5.3 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG 4000, CIT-PHOS, NACL, DTT, pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 293K |