2ODM

Crystal structure of S. aureus YlaN, an essential leucine rich protein involved in the control of cell shape

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX10.1
Synchrotron site	SRS
Beamline	PX10.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-26
Detector	MAR CCD 165 mm
Wavelength(s)	0.98000
Spacegroup name	P 1 21 1
Unit cell lengths	31.425, 42.771, 62.593
Unit cell angles	90.00, 92.42, 90.00

Refinement procedure

Resolution	15.000 - 2.240
R-factor	0.22659
Rwork	0.224
R-free	0.28317
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.031
Data reduction software	MOSFLM
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000
High resolution limit [Å]	2.240	2.240
Rmerge	0.079	0.429
<I/σ(I)>	10.5	2.6
Completeness [%]	100.0	99.7
Redundancy	3.6	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	0.2M Sodium acetate, 0.1M Tris-HCl pH 8.5, 20% PEG4000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	8	291	0.2M Sodium acetate, 0.1M Tris-HCl pH 8.5, 20% PEG4000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K