2ODL
Crystal structure of the HMW1 secretion domain from Haemophilus influenzae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-05 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97904 |
| Spacegroup name | I 4 |
| Unit cell lengths | 121.438, 121.438, 50.582 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.610 - 1.920 |
| R-factor | 0.17633 |
| Rwork | 0.174 |
| R-free | 0.21730 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.140 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.990 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.099 | 0.228 |
| Number of reflections | 28035 | |
| <I/σ(I)> | 6.2 | |
| Completeness [%] | 98.8 | 95.1 |
| Redundancy | 6.5 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 290 | 24% PEG6000, 0.1 M Tris-HCl, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
