2OD8
Structure of a peptide derived from Cdc9 bound to PCNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-06-02 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97900 |
Spacegroup name | I 2 3 |
Unit cell lengths | 139.280, 139.280, 139.280 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.700 - 2.800 |
R-factor | 0.24865 |
Rwork | 0.247 |
R-free | 0.28527 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1plq |
RMSD bond length | 0.007 |
RMSD bond angle | 1.081 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.039 | 0.418 |
Number of reflections | 11287 | |
<I/σ(I)> | 5 | 2 |
Completeness [%] | 99.2 | 99.2 |
Redundancy | 4.4 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 294 | 1.6 M (NH4)2SO4, Sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 294K |