2OD5

CRYSTAL STRUCTURE OF A PUTATIVE NUCLEIC ACID BINDING PROTEIN (JCVI_PEP_1096688149193) FROM UNCULTURED MARINE ORGANISM AT 1.79 A RESOLUTION

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-11-19
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837, 0.97971
Spacegroup name	P 62 2 2
Unit cell lengths	64.406, 64.406, 133.246
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.989 - 1.790
R-factor	0.19
Rwork	0.189
R-free	0.21300
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.418
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.989	28.990	1.840
High resolution limit [Å]	1.600	8.010	1.790
Rmerge	0.137	0.017	0.017
Total number of observations		2314	16140
Number of reflections	16175
<I/σ(I)>	3.9	9.9	0.4
Completeness [%]	100.0	97.5	100
Redundancy	13.6	9.8	14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP, NANODROP	7	277	1.0M LiCl, 20.0% PEG-6000, 0.1M HEPES pH 7.0, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K