2OD4

Crystal structure of a dimeric ferredoxin-like protein (jcvi_pep_1096665735785) from uncultured marine organism at 1.70 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-11-19
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837, 0.97966
Spacegroup name	P 1 21 1
Unit cell lengths	31.890, 77.790, 43.810
Unit cell angles	90.00, 102.66, 90.00

Refinement procedure

Resolution	28.892 - 1.700
R-factor	0.161
Rwork	0.159
R-free	0.19300
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.415
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.892	28.900	1.760
High resolution limit [Å]	1.700	3.660	1.700
Rmerge	0.061	0.040	0.403
Number of reflections	22797
<I/σ(I)>	6.76	13.73	2.02
Completeness [%]	98.3	96.5	99

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP, NANODROP	4.5	277	0.2M Li2SO4, 2.5M NaCl, 0.1M Acetate pH 4.5, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K