2OCY
Complex of the guanine exchange factor Sec2p and the Rab GTPase Sec4p
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 160 |
| Detector technology | CCD |
| Collection date | 2006-02-10 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 92.999, 92.999, 295.909 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.300 |
| R-factor | 0.25 |
| Rwork | 0.250 |
| R-free | 0.32500 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.077 | 0.305 |
| Number of reflections | 11817 | |
| <I/σ(I)> | 14.5 | 3.3 |
| Completeness [%] | 97.7 | 96.7 |
| Redundancy | 4.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 295 | 100 mM sodium acetate (pH 5.0), 200 mM calcium acetate, 20% PEG 400, 10mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
