2OCX

Crystal structure of Se-Met fucosyltransferase NodZ from Bradyrhizobium

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-20
Detector	MAR CCD 165 mm
Wavelength(s)	0.9537, 0.9787, 0.9790
Spacegroup name	P 65 2 2
Unit cell lengths	124.200, 124.200, 96.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.960 - 2.200
R-factor	0.186
Rwork	0.184
R-free	0.21700
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.532
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	Auto-Rickshaw
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.085	0.346
Number of reflections	22609
<I/σ(I)>	56	13.4
Completeness [%]	100.0	100
Redundancy	42.6	43.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	292	0.3M potassium dihydrogen phosphate, 0.1M Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K