2OCS
The crystal structure of the first PDZ domain of human NHERF-2 (SLC9A3R2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-26 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 26.556, 39.867, 37.278 |
| Unit cell angles | 90.00, 106.93, 90.00 |
Refinement procedure
| Resolution | 35.660 - 1.500 |
| R-factor | 0.15411 |
| Rwork | 0.152 |
| R-free | 0.19829 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | swissmodel generated model |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.941 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.660 | 1.600 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.066 | 0.460 |
| Number of reflections | 12057 | |
| <I/σ(I)> | 12.68 | 5.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.67 | 3.64 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 20% PEG 3350, 0.20M KSCN, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
