2OCH
J-domain of dnj-12 from Caenorhabditis elegans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-21 |
| Detector | SBC-3 |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 37.664, 51.758, 85.803 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.600 - 1.860 |
| R-factor | 0.2204 |
| Rwork | 0.219 |
| R-free | 0.24720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2O37 PDB ENTRY |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.566 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.500 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.052 | 0.601 |
| Number of reflections | 7363 | |
| <I/σ(I)> | 34.8 | 3.04 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.5 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 2.4 M sodium malonate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
