2OCE
Crystal structure of Tex family protein PA5201 from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-18 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.974, 113.027, 144.186 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.100 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.27900 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.280 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.173 | 0.573 |
| Number of reflections | 18579 | |
| <I/σ(I)> | 20 | 12.3 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 12.9 | 12.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 277 | 13% PEG 8000, 0.1M Sodium cacodylate pH 6.5, 0.12M Magnesium acetate, 5% Ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 277K, pH 6.50 |
