2OCD
Crystal structure of L-asparaginase I from Vibrio cholerae O1 biovar eltor str. N16961
Replaces: 2H7KExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-15 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.235, 117.838, 121.431 |
| Unit cell angles | 90.00, 91.44, 90.00 |
Refinement procedure
| Resolution | 33.300 - 2.450 |
| R-factor | 0.179 |
| Rwork | 0.176 |
| R-free | 0.24600 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.822 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.490 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.131 | 0.510 |
| Number of reflections | 55186 | |
| <I/σ(I)> | 10.3 | 2.2 |
| Completeness [%] | 97.4 | 93.1 |
| Redundancy | 3.8 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 291 | 2M Ammonium Acetate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K, pH 7.00 |
