2OCB

Crystal structure of human RAB9B in complex with a GTP analogue

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-08-29
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	70.430, 70.430, 97.715
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.051 - 2.200
Rwork	0.213
R-free	0.25310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wms
RMSD bond length	0.017
RMSD bond angle	1.416
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (refmac_5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.280
High resolution limit [Å]	2.200	4.730	2.200
Rmerge	0.107	0.042	0.725
Number of reflections	14718	1578	1440
<I/σ(I)>	10.2
Completeness [%]	99.9
Redundancy	6.1	5.9	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	2M ammonium sulfate, 0.2M sodium chloride, 0.1M HEPES, pH 7.5, vapor diffusion, hanging drop, temperature 291K