2OC6
Crystal structure of a protein from the duf1801 family (ydhg, bsu05750) from bacillus subtilis at 1.75 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-20 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97921, 0.97942 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 48.782, 46.903, 64.568 |
| Unit cell angles | 90.00, 110.05, 90.00 |
Refinement procedure
| Resolution | 37.113 - 1.750 |
| R-factor | 0.175 |
| Rwork | 0.173 |
| R-free | 0.21700 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.274 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 37.113 | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 3.770 | 1.750 |
| Rmerge | 0.078 | 0.052 | 0.322 |
| Number of reflections | 23448 | ||
| <I/σ(I)> | 10.9 | ||
| Completeness [%] | 85.1 | 99.7 | 40.2 |
| Redundancy | 3.2 | 3.5 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP, NANODROP | 277 | 32.0% polyethylene glycol 8000, 0.25M ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K |
