2OC3
Crystal Structure of the Catalytic Domain of Human Protein Tyrosine Phosphatase non-receptor Type 18
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-26 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.338, 63.763, 48.992 |
| Unit cell angles | 90.00, 102.64, 90.00 |
Refinement procedure
| Resolution | 38.240 - 1.500 |
| R-factor | 0.15557 |
| Rwork | 0.154 |
| R-free | 0.18395 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdb entries 2B49 1gwz |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.471 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.240 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.065 | 0.343 |
| Number of reflections | 44277 | |
| <I/σ(I)> | 12.4 | 3.4 |
| Completeness [%] | 99.3 | 95.2 |
| Redundancy | 3.7 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | 277 | 0.1M HEPES, 25% PEG-3350, 6% Jeffamine M-600, pH 6.8, temperature 277K |
