2OBY
Crystal structure of Human P53 inducible oxidoreductase (TP53I3,PIG3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-06-10 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 68.354, 184.359, 318.047 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.940 - 3.000 |
| R-factor | 0.22155 |
| Rwork | 0.220 |
| R-free | 0.25968 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j8z |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.935 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.940 | 3.200 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 36209 | |
| Completeness [%] | 88.7 | 62.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 30 % PEG 4000, 0.1 M Tris, 0.2 M sodium acetate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
