2OBP

Crystal structure of a putative dna-binding protein (reut_b4095) from ralstonia eutropha jmp134 at 1.70 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-03
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837, 0.97932, 0.97910
Spacegroup name	P 63 2 2
Unit cell lengths	76.580, 76.580, 137.860
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.412 - 1.700
R-factor	0.188
Rwork	0.186
R-free	0.21900
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.511
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.412		1.760
High resolution limit [Å]	1.700	2.900	1.700
Rmerge	0.063	0.042	0.628
Number of reflections	26996
<I/σ(I)>	19.02	41.3	3.1
Completeness [%]	98.2	99.7	90.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP, NANODROP	5.8	277	0.2M MgNO3, 20.0% PEG-3350, No Buffer pH 5.8, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K