2OBN

Crystal structure of a duf1611 family protein (ava_3511) from anabaena variabilis atcc 29413 at 2.30 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-08
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000, 0.9806, 0.9805
Spacegroup name	P 1 21 1
Unit cell lengths	75.090, 123.070, 75.210
Unit cell angles	90.00, 103.10, 90.00

Refinement procedure

Resolution	29.260 - 2.300
R-factor	0.177
Rwork	0.175
R-free	0.21400
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.464
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.260		2.380
High resolution limit [Å]	2.300	3.930	2.300
Rmerge	0.056	0.030	0.307
Number of reflections	58378
<I/σ(I)>	9.71	19.5	2.4
Completeness [%]	93.5	95.9	85.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP, NANODROP	7.2	277	0.2M NaFormate, 20.0% PEG-3350, No Buffer pH 7.2, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K