2OBL
Structural and biochemical analysis of a prototypical ATPase from the type III secretion system of pathogenic bacteria
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.973952 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 84.382, 77.609, 52.901 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 16.000 - 1.800 |
| R-factor | 0.19704 |
| Rwork | 0.195 |
| R-free | 0.23173 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.084 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.063 | 0.457 |
| Number of reflections | 32089 | |
| <I/σ(I)> | 182.3 | 3.18 |
| Completeness [%] | 98.8 | 95.1 |
| Redundancy | 15.3 | 11.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 7% PEG 8000, 0.1M Calcium Acetate, 0.1 M Imidazole, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
