2OBE
Crystal Structure of Chimpanzee Adenovirus (Type 68/Simian 25) Major Coat Protein Hexon
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-08 |
| Detector | BRANDEIS - B4 |
| Wavelength(s) | 1.100 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 90.800, 433.000, 159.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.270 - 2.100 |
| R-factor | 0.169 |
| Rwork | 0.169 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1RUX |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.828 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.089 | 0.253 |
| Number of reflections | 177830 | |
| <I/σ(I)> | 15.7 | 4.3 |
| Completeness [%] | 97.7 | 87.8 |
| Redundancy | 13.1 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 295 | 0.1 M sodium cacodylate, 0.2 M ammonium phosphate, 45% 2-methyl-2,4-pentanediol (MPD), pH 5.0, vapor diffusion, hanging drop, temperature 295K |
