2OBB

Structure of the conserved protein coded by locus BT_0820 from Bacteroides thetaiotaomicron

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-12-21
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97906, 0.97923
Spacegroup name	P 43 21 2
Unit cell lengths	50.898, 50.898, 129.386
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.340 - 2.200
R-factor	0.21382
Rwork	0.212
R-free	0.24475
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.437
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.340	2.260
High resolution limit [Å]	2.200	2.200
Number of reflections	8658
<I/σ(I)>	9
Completeness [%]	94.1	65.4
Redundancy	14.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.2M MgCl2, 0.1M Bis-Tris pH 6.5, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 291K