2OB9
Structure of bacteriophage HK97 tail assembly chaperone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-12 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | I 4 |
| Unit cell lengths | 101.140, 101.140, 63.382 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.800 - 2.300 |
| R-factor | 0.23484 |
| Rwork | 0.232 |
| R-free | 0.29138 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.677 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.800 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.062 | 0.402 |
| Number of reflections | 13384 | |
| <I/σ(I)> | 16.8 | 2.12 |
| Completeness [%] | 98.9 | 92.9 |
| Redundancy | 3.3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 298 | 0.2M NH4(OAc), 0.1M Na HEPES pH 7.6, 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
