2OB5
Crystal structure of protein Atu2016, putative sugar binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-06 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97904 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 63.322, 63.322, 71.382 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.350 - 1.600 |
| R-factor | 0.17853 |
| Rwork | 0.177 |
| R-free | 0.20575 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.453 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.095 | 0.578 |
| Number of reflections | 19728 | |
| <I/σ(I)> | 35.29 | 2.62 |
| Completeness [%] | 99.7 | 98.2 |
| Redundancy | 13 | 11.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.1M Hepes, 0.2M Ammonium Sulfate, 25% PEG3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
