2OB4

Human Ubiquitin-Conjugating Enzyme CDC34

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-12-18
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97917
Spacegroup name	I 2 2 2
Unit cell lengths	42.280, 66.310, 124.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.030 - 2.400
R-factor	0.22637
Rwork	0.223
R-free	0.28448
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.410
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MLPHARE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.450
High resolution limit [Å]	2.400	2.400
Number of reflections	7162
<I/σ(I)>	2.64	2.6
Completeness [%]	99.5	94.8
Redundancy	1.82	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	The protein was dissolved at 42 mg/ml in 20 mM Tris-HCl, pH 8.0, 0.15 M NaCl, 5% glycerol and 2 mM DTT. Crystals were grown in hanging drops by mixing 2 microL protein solution with 2 microL well solution (28% PEG 4000, 0.1 M Tris-HCl, pH 8.5, 0.2 M MgCl2, 1 mM DTT and 7.5 mM glycyl-glycyl-glycine) at 21 deg C. For cryoprotection, the crystals were soaked in the well solution supplemented with 25% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 298K