Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | I 41 |
Unit cell lengths | 212.308, 212.308, 97.680 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.500 |
R-factor | 0.319 |
Rwork | 0.319 |
R-free | 0.33800 |
Structure solution method | MIR |
RMSD bond length | 0.012 |
RMSD bond angle | 1.910 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.600 |
High resolution limit [Å] | 3.500 | 3.500 |
Number of reflections | 25597 | |
<I/σ(I)> | 12.6 | 1 |
Completeness [%] | 50.4 | |
Redundancy | 2.7 | 1.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 3.7 | PROTEIN CONCENTRATION 15-20 MG/ML AND 0.05% DODECYLMALTOSIDE.PROTEIN WAS MIXED IN A RATIO OF 4:3 OR 3:2 WITH THE RESERVOIR SOLUTION CONTAINING 100-120 mM AMMONIUM SULFUATE, 23-27% TRIETHYLENE GLYCOL, 100 mM GLYCINE, WITH 1-3 mM GD(CL)3 OR SM(CL)3 AND D2O AS THE SOLVENT. 1 mM (NA3)AU(S2O3)2 WAS SOAKED INTO THE CRYSTAL, pH 3.7, VAPOR DIFFUSION, SITTING DROP |