2OAP
Crystal structure of the archaeal secretion ATPase GspE in complex with AMP-PNP
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.96333,0.97912,0.97802 |
Spacegroup name | H 3 2 |
Unit cell lengths | 132.864, 132.864, 442.261 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.830 - 2.950 |
Rwork | 0.226 |
R-free | 0.23400 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.830 | 3.060 |
High resolution limit [Å] | 2.950 | 2.950 |
Rmerge | 0.106 | 0.478 |
Number of reflections | 32158 | |
<I/σ(I)> | 7.2 | 4.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.9 | 10.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.9 | 298 | 10% PEG 8000, 5% PEG 400, 1.5 M NaCl, 0.1 M Na/K phosphate, pH 8.9, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.9 | 298 | 10% PEG 8000, 5% PEG 400, 1.5 M NaCl, 0.1 M Na/K phosphate, pH 8.9, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.9 | 298 | 10% PEG 8000, 5% PEG 400, 1.5 M NaCl, 0.1 M Na/K phosphate, pH 8.9, VAPOR DIFFUSION, HANGING DROP, temperature 298K |