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2OAP

Crystal structure of the archaeal secretion ATPase GspE in complex with AMP-PNP

Experimental procedure
Experimental methodMAD
Source typeSYNCHROTRON
Source detailsSSRL BEAMLINE BL9-1
Synchrotron siteSSRL
BeamlineBL9-1
Temperature [K]100
Detector technologyCCD
Collection date2004-07-04
DetectorADSC QUANTUM 315
Wavelength(s)0.96333,0.97912,0.97802
Spacegroup nameH 3 2
Unit cell lengths132.864, 132.864, 442.261
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution49.830 - 2.950
Rwork0.226
R-free0.23400
Structure solution methodMAD
RMSD bond length0.007
RMSD bond angle1.400
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareSHARP
Refinement softwareCNS (1.1)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]49.8303.060
High resolution limit [Å]2.9502.950
Rmerge0.1060.478
Number of reflections32158
<I/σ(I)>7.24.5
Completeness [%]100.0100
Redundancy10.910.6
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP8.929810% PEG 8000, 5% PEG 400, 1.5 M NaCl, 0.1 M Na/K phosphate, pH 8.9, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1VAPOR DIFFUSION, HANGING DROP8.929810% PEG 8000, 5% PEG 400, 1.5 M NaCl, 0.1 M Na/K phosphate, pH 8.9, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1VAPOR DIFFUSION, HANGING DROP8.929810% PEG 8000, 5% PEG 400, 1.5 M NaCl, 0.1 M Na/K phosphate, pH 8.9, VAPOR DIFFUSION, HANGING DROP, temperature 298K

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