2OAL
RebH with bound FAD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-08 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.97496 |
Spacegroup name | P 62 |
Unit cell lengths | 114.725, 114.725, 230.880 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.900 - 2.100 |
Rwork | 0.197 |
R-free | 0.22200 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2e4g |
RMSD bond length | 0.007 |
RMSD bond angle | 1.250 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.900 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 99732 | |
<I/σ(I)> | 15.8 | 6.6 |
Completeness [%] | 99.6 | 97.2 |
Redundancy | 3.9 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 7 | 277 | 1.2 M Na/K phosphate, pH 7.0, hanging drop, temperature 277K |