2OAJ
Crystal structure of Sro7 from S. cerevisiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-07-18 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0781 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 130.520, 130.520, 116.023 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.120 - 2.400 |
R-factor | 0.211 |
Rwork | 0.211 |
R-free | 0.26200 |
Structure solution method | SIRAS |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SHARP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.300 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.108 | 0.430 |
Number of reflections | 39624 | |
<I/σ(I)> | 13.7 | 3.6 |
Completeness [%] | 99.8 | 100 |
Redundancy | 4.7 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 40 mM MES pH 6.5, 12% PEG 3350, 200 mM ammonium fluoride, VAPOR DIFFUSION, HANGING DROP, temperature 294K |