2OAI
The structure of transporter associated domain CorC_HlyC from a Xylella fastidiosa Temecula1 hemolysin.
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97924, 0.97938, 0.98706 |
Spacegroup name | I 2 3 |
Unit cell lengths | 87.905, 87.905, 87.905 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.080 - 1.800 |
R-factor | 0.17583 |
Rwork | 0.175 |
R-free | 0.19977 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.630 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.100 | 1.840 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 10567 | |
<I/σ(I)> | 13.1 | 2.1 |
Completeness [%] | 99.3 | 92.7 |
Redundancy | 8.6 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.05M CaCl2, 0.1M Bis-Tris pH 6.5, 30% PEG MME550, VAPOR DIFFUSION, SITTING DROP, temperature 291K |