2OA5
Crystal structure of ORF52 from Murid herpesvirus (MUHV-4) (Murine gammaherpesvirus 68) at 2.1 A resolution. Northeast Structural Genomics Consortium target MHR28B.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-09 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 93.462, 49.393, 56.379 |
Unit cell angles | 90.00, 111.88, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.100 |
R-factor | 0.215 |
Rwork | 0.215 |
R-free | 0.24100 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | COMO (1.2) |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 | 2.170 |
High resolution limit [Å] | 2.100 | 4.510 | 2.100 |
Rmerge | 0.064 | 0.051 | 0.353 |
Number of reflections | 13827 | ||
<I/σ(I)> | 18.8 | ||
Completeness [%] | 98.4 | 99.9 | 86.7 |
Redundancy | 7.1 | 6.6 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 80% PEG 400, 100MM MOPS, 100MM NANO3, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |