2O9V
The second SH3 domain from Ponsin in complex with the paxillin proline rich region
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8156 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 24.490, 41.852, 58.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.810 - 1.630 |
| R-factor | 0.1436 |
| Rwork | 0.143 |
| R-free | 0.17686 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o2w |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.608 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.660 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.059 | 0.221 |
| Number of reflections | 7747 | |
| <I/σ(I)> | 24 | 4.2 |
| Completeness [%] | 96.6 | 80.5 |
| Redundancy | 5 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 0.2M Sodium Acetate, 0.1M Sodium Cacodylate, 30% (w/v) PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
