2O9O
Crystal Structure of the buffalo Secretory Signalling Glycoprotein at 2.8 A resolution
Replaces: 1SV8Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 278 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-12-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.100, 66.859, 108.543 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.18843 |
| Rwork | 0.186 |
| R-free | 0.23619 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2esc |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.865 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 11349 | |
| <I/σ(I)> | 11 | 2.7 |
| Completeness [%] | 97.1 | 95.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | TRIS-HCL, NACL, 19% ETHANOL, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
