2O9F

Crystal Structure of AqpZ mutant L170C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Detector technology	CCD
Detector	ADSC QUANTUM 210
Spacegroup name	P 4
Unit cell lengths	91.333, 91.333, 77.504
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	91.290 - 2.550
R-factor	0.238
Rwork	0.236
R-free	0.28000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.452
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	91.290
High resolution limit [Å]	2.550
Number of reflections	15207
Completeness [%]	74.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5		hanging drop with 1:1 addition of protien and mothor liquor, 25% polyethylene glycol monomethyl ether 2000, 100 mM sodium cacodylate, 100 mM MgCl2, pH 6.5, VAPOR DIFFUSION, HANGING DROP
1	VAPOR DIFFUSION, HANGING DROP	6.5		hanging drop with 1:1 addition of protien and mothor liquor, 25% polyethylene glycol monomethyl ether 2000, 100 mM sodium cacodylate, 100 mM MgCl2, pH 6.5, VAPOR DIFFUSION, HANGING DROP