2O9D
Crystal Structure of AqpZ mutant T183C.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 4 |
| Unit cell lengths | 92.428, 92.428, 78.235 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 92.450 - 2.300 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.647 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 92.450 | 92.450 | 2.420 |
| High resolution limit [Å] | 2.300 | 7.270 | 2.300 |
| Rmerge | 0.073 | 0.033 | 0.595 |
| Total number of observations | 3339 | 11355 | |
| Number of reflections | 28901 | ||
| <I/σ(I)> | 6.6 | 16.5 | 1.2 |
| Completeness [%] | 98.3 | 96.4 | 93.8 |
| Redundancy | 3.3 | 3.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | hanging drop with 1:1 addition of protien and mothor liquor, 25% polyethylene glycol monomethyl ether 2000, 100 mM sodium cacodylate, 100 mM MgCl2, pH 6.5, VAPOR DIFFUSION, HANGING DROP |
