2O9C
Crystal Structure of Bacteriophytochrome chromophore binding domain at 1.45 angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 5ID-B |
Synchrotron site | APS |
Beamline | 5ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-25 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 89.333, 51.750, 80.437 |
Unit cell angles | 90.00, 116.29, 90.00 |
Refinement procedure
Resolution | 19.550 - 1.450 |
R-factor | 0.1664 |
Rwork | 0.165 |
R-free | 0.19204 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ZTU without chromophore or his tag |
RMSD bond length | 0.012 |
RMSD bond angle | 1.842 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.500 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.039 | 0.276 |
Number of reflections | 56868 | |
<I/σ(I)> | 12.6 | 3 |
Completeness [%] | 97.5 | 83.3 |
Redundancy | 3.7 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 0.095 M sodium citrate, 19% v/v isopropanol, 19% v/v PEG 4000, 5% V/V glycerol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |