2O99
The crystal structure of E.coli IclR C-terminal fragment in complex with glyoxylate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-02-16 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.538, 81.483, 154.693 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.700 |
R-factor | 0.20661 |
Rwork | 0.204 |
R-free | 0.26116 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.023 |
RMSD bond angle | 1.861 |
Data reduction software | d*TREK |
Data scaling software | d*TREK (9.2SSI) |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.590 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.060 | 0.352 |
Number of reflections | 73139 | |
<I/σ(I)> | 13.7 | 3.9 |
Completeness [%] | 97.1 | 85.5 |
Redundancy | 5.93 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 297 | 0.2M Potassium Acetate, pH 7.0, 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 297K |