2O95

Crystal Structure of the Metal-Free Dimeric Human Mov34 MPN domain (residues 1-186)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE D03B-MX1
Synchrotron site	LNLS
Beamline	D03B-MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-06
Detector	MAR CCD 165 mm
Wavelength(s)	1.427
Spacegroup name	C 1 2 1
Unit cell lengths	114.103, 95.289, 58.988
Unit cell angles	90.00, 121.13, 90.00

Refinement procedure

Resolution	30.210 - 1.950
R-factor	0.203
Rwork	0.201
R-free	0.24000
Structure solution method	SAD
RMSD bond length	0.021
RMSD bond angle	2.050
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.205	2.060
High resolution limit [Å]	1.950	1.950
Rmerge	0.050	0.476
Total number of observations		17623
Number of reflections	39272
<I/σ(I)>	11.9	1.6
Completeness [%]	99.7	99.5
Redundancy	3.3	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	291	0.1M phosphate-citrate buffer, 20% PEG 1000, 0.2M LiSO4, 10mM MnCl2 as additive, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	4.2	291	0.1M phosphate-citrate buffer, 20% PEG 1000, 0.2M LiSO4, 10mM MnCl2 as additive, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K