2O95
Crystal Structure of the Metal-Free Dimeric Human Mov34 MPN domain (residues 1-186)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE D03B-MX1 |
Synchrotron site | LNLS |
Beamline | D03B-MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-06 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.427 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 114.103, 95.289, 58.988 |
Unit cell angles | 90.00, 121.13, 90.00 |
Refinement procedure
Resolution | 30.210 - 1.950 |
R-factor | 0.203 |
Rwork | 0.201 |
R-free | 0.24000 |
Structure solution method | SAD |
RMSD bond length | 0.021 |
RMSD bond angle | 2.050 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.205 | 2.060 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.050 | 0.476 |
Total number of observations | 17623 | |
Number of reflections | 39272 | |
<I/σ(I)> | 11.9 | 1.6 |
Completeness [%] | 99.7 | 99.5 |
Redundancy | 3.3 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 291 | 0.1M phosphate-citrate buffer, 20% PEG 1000, 0.2M LiSO4, 10mM MnCl2 as additive, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 291 | 0.1M phosphate-citrate buffer, 20% PEG 1000, 0.2M LiSO4, 10mM MnCl2 as additive, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |