2O92
Crystal structure of a signalling protein (SPG-40) complex with tetrasaccharide at 3.0A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-12-01 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.638, 66.238, 107.158 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.900 - 3.000 |
| R-factor | 0.1828 |
| Rwork | 0.181 |
| R-free | 0.22371 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2aos |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.001 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.900 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 8096 | |
| Completeness [%] | 99.0 | 97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | Tris-HCl, 19% Ethanol, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
