2O8S
X-ray Crystal Structure of Protein AGR_C_984 from Agrobacterium tumefaciens. Northeast Structural Genomics Consortium Target AtR120.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 43 |
| Unit cell lengths | 42.490, 42.490, 332.804 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.730 - 2.400 |
| R-factor | 0.2428 |
| Rwork | 0.243 |
| R-free | 0.27930 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.730 | 2.480 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.760 | 0.365 |
| Number of reflections | 46946 | |
| <I/σ(I)> | 18.1 | 3.5 |
| Completeness [%] | 99.0 | 97.4 |
| Redundancy | 5.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10 | 291 | 100mM CAPS, 40% PEG1k, 100mM ammonium sulfate, 5mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
