2O8O
Crystal structure of Clostridium histolyticum colg collagenase collagen-binding domain 3B at 1.35 Angstrom resolution in presence of calcium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Wavelength(s) | 1.28149 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.866, 59.170, 48.801 |
| Unit cell angles | 90.00, 100.77, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.350 |
| R-factor | 0.1469 |
| Rwork | 0.147 |
| R-free | 0.20980 |
| Structure solution method | AB INITIO |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.032 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | |
| High resolution limit [Å] | 1.350 | 1.350 |
| Number of reflections | 45040 | |
| <I/σ(I)> | 1.8 | |
| Completeness [%] | 99.8 | 99.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | 25-27% PEG 3350, 100mM Sodium acetate, 600mM LiCl, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
