2O8L
Structure of V8 protease from staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Detector technology | CCD |
| Collection date | 2006-08-04 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.3317 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 59.430, 59.430, 214.010 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.370 - 1.500 |
| R-factor | 0.1899 |
| Rwork | 0.188 |
| R-free | 0.21867 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qy6 |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.366 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.400 | 1.630 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.130 | 0.418 |
| Number of reflections | 58412 | |
| <I/σ(I)> | 9.95 | 3.2 |
| Completeness [%] | 89.8 | 92.3 |
| Redundancy | 5.94 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 293 | PEG 5000 MME, KCl, HEPES, pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
